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Bacterium – fungus interactions during growth substrate colonisation and fruiting body formation of oyster mushroom (Pleurotus ostreatus)

Identifier of application: 
OTKA K 83764
Supervisor: 
Károly Márialigeti
Participants: Balázs Vajna, Bánfi Renáta, Zsuzsa Pohner, Károly Bóka, Gábor M. Kovács, Pál Vági
Period: 
2011-02-01 - 2016-01-31
Summary: 

 

Pleurotus ostreatus, being a saprotrophic white rot fungus, is a good model organism for studying bacterium – fungus interactions, because its participation in antagonistic and synergistic interactions has been demonstrated earlier. Pleurotus sp. is an important commercial mushroom; in the latest times it became involved in waste-management and bioremediation studies, too. Though little is known yet on the nature and succession of bacterium - fungus interactions during growth-substrate colonization and fruiting body formation, and especially of their interplay in lignocellulose degradation. Last but not least most of the results presented yet were obtained from experiments carried out only under axenic agar plate culture conditions using traditional laboratory media.
The aim of the present study is to get deeper insight into to the bacterium – fungus interplays during the colonization of mushroom substrate and fruiting body formation in a laboratory model system using native composted oyster mushroom growth substrate. Following parameters will be monitored: structure and lignocellulolytic enzymatic profile of oyster mushroom hyphae; composition and activity of the bacterial community in the substrate and in the hyphoplan/hyphosphere (FISH, T-RFLP, clone-libraries); structural changes at fruiting body primordium formation; hypothetical intracellular colonization of hyphae and fruiting body. The results will be compared with controls using prior to spawning sterilized substrate, etc.
Through this basic research better understanding of the interactions between Pleurotus ostreatus and bacteria will be obtained. During the planned studies the research group shall shapen important methodological (e.g. hyphosphere preparation, special fluorescence techniques) and infrastructural (phytotron, diamond knife) developments.