Tóth, E.M., Kéki, Zs., Homonnay, Z.G., Borsodi, A.K., Márialigeti, K., Schumann, P. 2008. Nocardioides daphniae sp. nov., isolated from Daphnia cucullata (Crustacea: Cladocera). International Journal of Systematic and Evolutionary Microbiology, 58, 78-83.

A Gram-positive, rod-shaped or coccoid, yellow-pigmented bacterial strain, D287(T), was isolated from the water flea Daphnia cucullata (Crustacea: Cladocera) collected from Lake Balaton in Hungary. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparisons revealed that the strain represented a distinct lineage within the cluster of the genera Nocardioides and Marmoricola. The following characteristics were consistent with the affiliation of strain D287(T) to the genus Nocardioides: peptidoglycan based on LL-2,6-diaminopimelic acid, MK-8(H(4)) as the major menaquinone, iso-C(16:0) as the predominant cellular fatty acid, the presence of phosphatidylglycerol and diphosphatidylglycerol and a DNA G+C content of 69.9 mol%. Owing to characteristic differences in physiological traits and levels of 16S rRNA gene sequence similarity to its phylogenetically closest neighbours that were below 97%, strain D287(T) is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides daphniae sp. nov. is proposed. The type strain is D287(T) (=DSM 18664(T)=CCM 7403(T)).

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