Rusznyák, A., Tóth, E.M., Schumann, P., Sproer, C., Makk, J., Szabó, G., Vladár, P., Márialigeti, K., Borsodi, A.K. 2011. Cellulomonas phragmiteti sp. nov., a cellulolytic bacterium isolated from reed (Phragmites australis) periphyton in a Hungarian shallow soda pond. International Journal of Systematic and Evolutionary Microbiology, 61, 1662-1666.
An alkalitolerant and moderately halophilic strain designated KB23T, characterized by optimal growth at pH 8.0–9.0 and at 5–7 % (w/v) NaCl, was isolated from a reed (Phragmites australis) periphyton sample originating from an extremely shallow, alkaline soda pond located in Hungary. Cells of strain KB23T are Gram-positive, motile straight rods. The strain KB23T is facultatively anaerobic, catalase-positive, oxidasenegative and contains peptidoglycan of type A4beta L-Orn - D-Asp. In strain KB23T MK-9(H4) is the predominant isoprenoid quinone and ai-C15:0, C16:0, ai-C15:1 are the major cellular fatty acids. The DNA G+C content of strain KB23T is 74.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belongs to the genus Cellulomonas and that it is most closely related to C. flavigena DSM 20109T (97.35 %), C. terrae DB5T (96.81 %), C. iranensis OT (96.75), C. chitinilytica X.bubT (96.60 %), C. persica  IT (96.53 %), C. composti TR7-06T (96.45 %), C. biazotea DSM 20112T (96.34 %) and C. fimi DSM 20113T (96.20 %). According to the results of DNA–DNA hybridization and physiological data, the  strain represents a novel species, for which the name Cellulomonas phragmiteti sp. nov. is proposed. The type strain is KB23T (=DSM 22512T =NCAIM B002303T).
Impact factor: