Máthé, I., Táncsics, A., György, E., Pohner, Z., Vladár, P., Székely, A.J., Márialigeti, K. 2010. Investigation of mineral water springs of Miercurea Ciuc (Csikszereda) region (Romania) with cultivation-dependent microbiological methods. Acta Microbiologica et Immunologica Hungarica, 57, 109-122.

Water samples of ten mineral water springs at Miercurea Ciuc (Csikszereda) region (Romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. The results of standard hygienic bacteriological tests showed that the Hargita Spring had perfect and five other springs had microbiologically acceptable water quality (Zsogod-, Nagy-borviz-, Taploca-, Szentegyhaza- and Lobogo springs). The water of Borsaros Spring was exceptionable (high germ count, presence of Enterococcus spp.). Both standard bacteriological and molecular microbiological methods indicated that the microbiological water quality of the Szeltersz-, Nadasszek- and Delo springs was not acceptable. Bad water quality resulted from inadequate spring catchment and hygiene (low yield, lack of runoff, negligent usage of the springs, horse manure around the spring). The 16S rRNA gene-based identification of strains isolated on standard meat-peptone medium resulted in the detection of typical aquatic organisms such as Shewanella baltica, Aeromonas spp., Pseudomonas veronii, Psychrobacter sp,. Acinetobacter spp. and allochthonous microbes, like Nocardia, Streptomyces, Bacillus, Microbacterium, and Arthrobacter strains indicating the impact of soil. Other allochthonous microbes, such as Staphylococcus spp., Micrococcus sp., Lactococcus sp., Clostridium butyricum, Yersinia spp., Aerococcus sp., may have originated from animal/human sources.